Thy-1 and LAT clusters occur on membrane regions without distinctive features. External cross-linking of Thy-1 promotes coclustering of Thy-1 with LAT, but not with GM1. ![]() Immunogold labeling and electron microscopy shows there is little or no colocalization of the raft markers Thy-1, GM1, and LAT with each other or with FcϵRI on native membrane sheets prepared from unstimulated cells. This study addresses the relationship of fractionation data to the organization of raft markers in native membranes. ![]() Lipid rafts isolated by detergent extraction and sucrose gradient fractionation from mast cells are enriched for the glycosylphosphatidylinositol-linked protein Thy-1, the ganglioside GM1, palmitoylated LAT, and cross-linked IgE receptors, FcϵRI.
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